Catalytic Activity and Inhibition of Wegener’s Antigen Proteinase 3 on the Cell Surface of Human Polymorphonuclear Neutrophils
نویسندگان
چکیده
1 INSERM U618 « Protéases et Vectorisation Pulmonaires » ; Université François Rabelais de Tours. 2 CHRU de Tours ; 3 INSERM U921 « Nutrition, Croissance et Cancer » ; 4 Institut Fédératif de Recherche 135 ; * Current address: Department of Neuroimmunology, Max Planck-Institute of Neurobiology, D-82152, Planegg-Martinsried, Germany Running head: Membrane-bound Proteinase 3 Address correspondence to: Francis Gauthier, Faculty of Medicine, 10 Bd Tonnellé, 37032 Tours Cedex, France. Tel.: (33) 2 47 36 60 45 ; Fax: (33) 2 47 36 60 46 ; E-mail: [email protected]
منابع مشابه
Catalytic activity and inhibition of wegener antigen proteinase 3 on the cell surface of human polymorphonuclear neutrophils.
Proteinase 3 (Pr3), the main target of anti-neutrophil cytoplasmic antibodies, is a neutrophil serine protease that may be constitutively expressed at the surface of quiescent circulating neutrophils. This raises the question of the simultaneous presence in the circulation of constitutive membrane-bound Pr3 (mPr3) and its plasma inhibitor alpha1-protease inhibitor (alpha1-Pi). We have looked at...
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Although proteinase 3 (PR3) is known to have the potential to promote inflammation and injure tissues, the biologic forms and function of PR3 in polymorphonuclear neutrophils (PMN) from healthy donors have received little attention. In this paper, we show that PMN contain 3.24 +/- SD 0.24 pg of PR3 per cell, and that the mean concentration of PR3 in azurophil granules of PMN is 13.4 mM. Low lev...
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Proteinase 3(PR3) is a human polymorphonuclear leukocyte serine proteinase and is the main target antigen for antineutrophil cytoplasmic antibodies (ANCA) found in Wegener's granulomatosis (WG). We developed a stable expression system for conformationally intact recombinant PR3 (rPR3) in Chinese hamster ovary cells (CHO-cells). The part of PR3 cDNA that encoded the active form of PR3 was s...
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